Isolation and molecular characterization of microbial isolates from Saryu river water

Authors

  • Sadanand Maurya Department of Botany, K.S. Saket P.G. College, Ayodhya, Uttar Pradesh, India
  • Manikant Tripathi Biotechnology Program, Dr. Rammanohar Lohia Avadh University, Ayodhya, Uttar Pradesh, India
  • Karunesh K. Tiwari Jhunjhunwala P.G. College, Hansapur, Dwarika Puri, Ayodhya, Uttar Pradesh, India
  • Awadhesh K. Shukla Department of Botany, K.S. Saket P.G. College, Ayodhya, Uttar Pradesh, India

DOI:

https://doi.org/10.58414/SCIENTIFICTEMPER.2023.14.4.13

Abstract

This study was aimed to investigate molecular characterization of microbial isolates likes bacteria, fungi using culture dependent approach and analyse the phylogenetic relationship between the isolates. The identification of bacteria and fungi was confirmed by DNA sequencing by PCR amplification using 16S rRNA and Internal Transcribed Spacer (ITS) genes, respectively. The DNA sequences of bacterial and fungal isolates were identified using Nucleotide Basic Alignment Search Tools (nBLAST) against the data available in GenBank. On the basis of retrieved DNA sequences from database phylogenetic trees were constructed and phylogenetic relationship among the bacterial and fungal isolates were computed.

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Published

27-12-2023

Issue

Vol. 14 No. 04 (2023): The Scientific Temper

Section

SECTION A: BIOLOGICAL SCIENCES, AGRICULTURE, BIOTECHNOLOGY, ZOOLOGY

License

Copyright (c) 2023 The Scientific Temper

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

How to Cite

Isolation and molecular characterization of microbial isolates from Saryu river water. (2023). The Scientific Temper, 14(04), 1142-1147. https://doi.org/10.58414/SCIENTIFICTEMPER.2023.14.4.13

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