Effect of Growth Regulators on Direct Clonal Propagation and Analysis of Total Phenolic Content of Wild and Propagated Mucuna pruriens

Authors

  • Neha Saini Department of Biotechnology, School of Basic & Applied Sciences, Shri Guru Ram Rai University, Patel Nagar, Dehradun-248001, Uttarakhand, India.
  • Rashmi Verma Department of Biotechnology, School of Basic & Applied Sciences, Shri Guru Ram Rai University, Patel Nagar, Dehradun-248001, Uttarakhand, India.
  • Rabia Basri Aziz Department of Biotechnology, School of Basic & Applied Sciences, Shri Guru Ram Rai University, Patel Nagar, Dehradun-248001, Uttarakhand, India.
  • Ashmita Bhatt Department of Biotechnology, School of Basic & Applied Sciences, Shri Guru Ram Rai University, Patel Nagar, Dehradun-248001, Uttarakhand, India.
  • Hem Chandra Pant Department of Biotechnology, School of Basic & Applied Sciences, Shri Guru Ram Rai University, Patel Nagar, Dehradun-248001, Uttarakhand, India.
  • Naveen Gaurav Department of Biotechnology, School of Basic & Applied Sciences, Shri Guru Ram Rai University, Patel Nagar, Dehradun-248001, Uttarakhand, India.

DOI:

https://doi.org/10.58414/SCIENTIFICTEMPER.2022.13.2.51

Keywords:

Auxiliary bud, Acclimatization, Cytokinin, Multiple shoots, Rapid micropropagation.

Abstract

Mucuna pruriens is an important vegetable cover crop. Almost every part of the plant is thought to contain L-3,4  to contain L-3,4 dihydroxyphenylalanine (L-Dopa). The present study describes a quick and dependable technique for high-constancy miniature spread. Helper nodal explants were refined on Murashige and Skoog’s (MS) media boosted with different groupings of cytokinins from 15-day-old refined explants. Throughout the primary culture on 35 to 45 mg/l 6-benzyl amino purine (BAP) greatest no of shoots was delivered. The number of shoots expanded when the flawless shoots were exposed to re-refine on a similar hormonal medium. The stretched shoots created a limit of roots on half-strength MS medium enhanced with 20-40 mg/l naphthalene acidic corrosive (NAA). The Plantlets were adjusted by moving them first to peat greenery: manure (1:1) combination further with sand: soil (1:1) blend, recording 90% endurance. This framework gives high constancy miniature engendering framework for proficient and fast miniature proliferation and evaluation of phenolic content of this significant green compost cover crop with restorative properties.

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Published

12-12-2022

How to Cite

Effect of Growth Regulators on Direct Clonal Propagation and Analysis of Total Phenolic Content of Wild and Propagated Mucuna pruriens. (2022). The Scientific Temper, 13(02), 354-359. https://doi.org/10.58414/SCIENTIFICTEMPER.2022.13.2.51

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