Effect of Growth Regulators on Direct Clonal Propagation and Analysis of Total Phenolic Content of Wild and Propagated Mucuna pruriens

Published

12-12-2022

DOI:

https://doi.org/10.58414/SCIENTIFICTEMPER.2022.13.2.51

Keywords:

Auxiliary bud, Acclimatization, Cytokinin, Multiple shoots, Rapid micropropagation.

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Authors

  • Neha Saini Department of Biotechnology, School of Basic & Applied Sciences, Shri Guru Ram Rai University, Patel Nagar, Dehradun-248001, Uttarakhand, India.
  • Rashmi Verma Department of Biotechnology, School of Basic & Applied Sciences, Shri Guru Ram Rai University, Patel Nagar, Dehradun-248001, Uttarakhand, India.
  • Rabia Basri Aziz Department of Biotechnology, School of Basic & Applied Sciences, Shri Guru Ram Rai University, Patel Nagar, Dehradun-248001, Uttarakhand, India.
  • Ashmita Bhatt Department of Biotechnology, School of Basic & Applied Sciences, Shri Guru Ram Rai University, Patel Nagar, Dehradun-248001, Uttarakhand, India.
  • Hem Chandra Pant Department of Biotechnology, School of Basic & Applied Sciences, Shri Guru Ram Rai University, Patel Nagar, Dehradun-248001, Uttarakhand, India.
  • Naveen Gaurav Department of Biotechnology, School of Basic & Applied Sciences, Shri Guru Ram Rai University, Patel Nagar, Dehradun-248001, Uttarakhand, India.

Abstract

Mucuna pruriens is an important vegetable cover crop. Almost every part of the plant is thought to contain L-3,4  to contain L-3,4 dihydroxyphenylalanine (L-Dopa). The present study describes a quick and dependable technique for high-constancy miniature spread. Helper nodal explants were refined on Murashige and Skoog’s (MS) media boosted with different groupings of cytokinins from 15-day-old refined explants. Throughout the primary culture on 35 to 45 mg/l 6-benzyl amino purine (BAP) greatest no of shoots was delivered. The number of shoots expanded when the flawless shoots were exposed to re-refine on a similar hormonal medium. The stretched shoots created a limit of roots on half-strength MS medium enhanced with 20-40 mg/l naphthalene acidic corrosive (NAA). The Plantlets were adjusted by moving them first to peat greenery: manure (1:1) combination further with sand: soil (1:1) blend, recording 90% endurance. This framework gives high constancy miniature engendering framework for proficient and fast miniature proliferation and evaluation of phenolic content of this significant green compost cover crop with restorative properties.

How to Cite

Saini, N., Verma, R., Aziz, R. B., Bhatt, A., Pant, H. C., & Gaurav, N. (2022). Effect of Growth Regulators on Direct Clonal Propagation and Analysis of Total Phenolic Content of Wild and Propagated Mucuna pruriens. The Scientific Temper, 13(02), 354–359. https://doi.org/10.58414/SCIENTIFICTEMPER.2022.13.2.51

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