Analytical Method Development and Validation Analysis for Quantitative Assessment of Thifluzamide by HPLC Procedure
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https://doi.org/10.58414/SCIENTIFICTEMPER.2022.13.1.21Keywords:
Thifluzamide , Robust, Precision, Linearity and Stability.Dimensions Badge
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The precise, systematic, explicit, particular, linear, exact and robust scientific method was developed and validated for the assay of Thifluzamide in THIFLUZAMIDE 24% SC(CILPYROX) fungicide. Presently utilized Thifluzamide as a working standard having limit f assay of Thifluzamide in THIFLUZAMIDE 24% SC (CILPYROX) fungicide are not less than 95.0%. Acetonitrile, water and Phosphoric acid in the ratio (60:40:0.1 v/v/v) used as mobile phase and flow rate 1.0 ml / min. with 10 minutes run time. The detection was carried at 230 nm with column c18 - 250mm x 4.6mm x 5μ and ambient column temperature was maintained. The linearity of this method was found to be linear with a coefficient of regression at 0.999 in the concentration range of 50% to 150%. The linear regression equation was y=2174x-135.8. The present developed HPLC method is detected to be suitable. The analytical solution was detected to be stable up to 48 Hrs at room temperature.Abstract
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