GC-MS Profiling and Analysis of Bioprotective Properties of Terminalia chebula against Non-Fermenting Gram-Negative Bacteria Isolated from Tertiary Care Hospital

Abstract

Due to the extensive use of commercially available antibiotics, there is an emergence of NFGNBs as nosocomial pathogens. The major concern is the adoption of multi-drug resistance among them which limits their alternatives for treatment. Nowadays, great emphasis is on exploring the medicinal efficacy of traditional plants for combating the infections caused by human pathogens. Terminalia chebula possesses several significant phyto-ingredient such as tannins and chebulinic acid which have been well reported to exhibit high antimicrobial and antioxidant activities. The present study aims on combating NFGNB infections with the various solvent extracts of Terminalia chebula. Isolation and characterization of NFGNB pathogens were done. T. chebula extracts were prepared in a series of different organic and water solvents based on increasing polarity index {Ethyl acetate (4.4), Methanol (5.1), and Water (9.0)} using the Soxhlet extraction method. Antibacterial activity of the crude plant extracts was determined by DPPH assay. GC-MS and HPLC analysis revealed the presence of a significant bioactive compound. The results demonstrate the strong antimicrobial activity and effective MIC, MBC, and MIC index values of the S1, S2, and S3 extract of T. Chebula against isolated NFGNB pathogens. Inhibitory activities of extracts were compared with the standard antibiotic, colistin. The medicinal plants have been regarded as valuable and cheap sources of various phytoconstituents which are used extensively in the development of drugs against various diseases.