In-vitro study on the antidiabetic property of Pisonia grandis

Abstract

The prevalence of diabetes seems to be growing, and many people report unfavorable reactions to currently available antidiabetic drugs. The use of ethnomedicinal plants has the potential to provide cost-effective and safe alternatives to conventional diabetic treatments. This in-vitro investigation aims to investigate the antidiabetic properties of Pisonia grandis. The extract’s cytotoxic potential on HepG2 cells was evaluated using the MTT assay. Additionally, it investigated its impact on glucose metabolism in HepG2 cells and its ability to inhibit enzymes like alpha-amylase and alpha-glucosidase. Both doses of plant extract caused less than 50% cell death. The extract was more effective than berberine in enhancing glucose uptake by “HepG2 liver cells”. When compared to the positive controls, the crude extract did not affect alpha-amylase, protein glycation, collagenase, alpha-glucosidase, DPP-IV, or lipase. P. grandis exhibits hypoglycaemic effects that may operate independently of insulin, suggesting its potential as an antidiabetic agent.