Analytical Method Development and Validation Analysis for Quantitative Assessment of Thifluzamide by HPLC Procedure

Abstract

The precise, systematic, explicit, particular, linear, exact and robust scientific method was developed and validated for the assay of Thifluzamide in THIFLUZAMIDE 24% SC(CILPYROX) fungicide. Presently utilized Thifluzamide as a working standard having limit f  assay of Thifluzamide in THIFLUZAMIDE 24% SC (CILPYROX) fungicide are not less than 95.0%. Acetonitrile, water and Phosphoric acid in the ratio (60:40:0.1 v/v/v) used as mobile phase and flow rate 1.0 ml / min. with 10 minutes run time. The detection was carried at 230 nm with column c18 - 250mm x 4.6mm x 5μ and ambient column temperature was maintained. The linearity of this method was found to be linear with a coefficient of regression at 0.999 in the concentration range of 50% to 150%. The linear regression equation was y=2174x-135.8. The present developed HPLC method is detected to be suitable. The analytical solution was detected to be stable up to 48 Hrs at room temperature.