SMOKE-WATER IMPROVES SHOOT GROWTH AND INDIGO ACCUMULATION IN SHOOTS OF INDIGO LINIFOLIA SEEDLINGS

Abstract

There is scarcity on researches about the effect of smoke on secondary metabolite production in plants, although smoke treatments have successfully been used for promoting the germination of many seeds, This study highlights the effects of smoke-water on shoot growth and accumulation of indigo in shoots of Indigo linifolia plant. Results showed that seedlings treated with smoke-water at a dilution of 1:1000 achieved the highest fresh  and dry shoot mass, which was significantly (PÂ0.05) different from the control and other smoke-water dilutions tested. A higher concentration of smoke-water (1:500) significantly increased (PÂ0.05) the indigo concentration in shoots compared with untreated seedlings. The effect of 1:1000 and 1:2000 dilutions gave nonsignificant increases in the concentration of indigo. These findings suggest the possible use of smoke-water for promoting the growth and accumulation
of secondary metabolites in plants