The impact of bone substitute combined with blood cell progenerators on the healing of surgical bony defects

Abstract

Five local breed male sheep have been used in the study. Four 7-mm diameter, 4-mm deep bone defects were intentionally drilled in each sheep’s tibia/radius for experimentation. General anesthesia surgeries were spaced 2 to 4 weeks apart. The first distal defect was left untreated as a control, whilst the second defect was subjected to a treatment involving a combination of standard-platelet-rich fibrin (S-PRF) and osteon III (hydroxyapatite+ Beta tricalcium phosphate (HA/β-TCP)). The third defect was treated with Advanced-PRF (A-PRF) and osteon III, while the fourth defect was treated with concentrated growth factors (CGFs) and osteon III. Two weeks following the final surgery, each sheep was sacrificed. Immunohistochemical analyses were performed. Immunohistochemistry revealed a statistically significant reaction to Ki-67 and osteopontine in the CGFs, especially at the end of weeks six and eight while no significant reactions to CD31 and CD34 antibodies at all study intervals.