ISOLATION AND SCREENING OF XYLANASE PRODUCING ASPERGILLUS SP FROM SOIL.

Abstract

Present investigation deals with the isolation and screening of Aspergillus sp. for the biosynthesis of xylanase. Isolation of fungi was carried out on Potato Dextrose Agar (PDA) followed by serial dilution method using the samples obtained from the soil collected
from different areas of Meerut. The culture was incubated at 30 0C for 3-5 days. The fungal isolates were subculture to purity and examine for xylanolytic activities. Screening for xylanolytic activities was also performed on potato dextrose agar (PDA) containing 0.1% (w/v) of xylan from oat spelt. Positive xylanolytic isolates were detected based on the clear zones of hydrolysis on the xylan. The fungi were considered forxylanase production.