Phytochemical Profiling and GCMS Analysis of Two Different Varieties of Barley (Hordeum vulgare L.) Under Fluoride Stress

Abstract

Hordeum vulgare L.(Barley) is an important staple crop in Asia and usually considered more fluoride tolerant than other cereal crops under family Poaceae. In this study, phytochemical and GCMS profiling of two cultivars of Hordeum vulgare L. viz.., RD2794 and RD2786 have been performed under Fluoride stress. Different concentrations of NaF (Control, 5ppm, 10 ppm, 15ppm, and 20 ppm) were prepared to identify cultivars responsible for tolerance to fluoride stress. Several strategies were identified in response to fluoride stress among the two cultivars. This paper reports the cultivars of fluoride adaptation between the two cultivars. RD2786 contains high alkaloids, carbohydrates, Phytosterols and fixed oil better than RD2794 after imposing fluoride stress. The study showed that total flavonoids and phenolic content is high in RD2786 and reduced significantly in RD2794. This suggests that RD2786 is a desired crop to cultivate under fluoride stress. Through the course of this study on the basis of several important parameters the fluoride tolerant varieties and susceptible varieties will be known. This outcome of the proposed research work may also help the plant breeders to produce resistanace against fluoride stress of barley.