Effect of Genotype and Explant on Shoot Regeneration in Brassica juncea

Abstract

In order to utilize biotechnological approaches for the improvement of B. juncea, a high frequency reproducible protocol for complete plantlet regeneration is required. With this aim cotyledon and plumule explants derived from 5 day old seedlings of five different cultivars of B. juncea were cultured on MS medium supplemented with 1.0 mg/l BAP and 0.2 mg/l IAA. Varuna exhibited significantly higher frequency (85%) of shoot regeneration than other genotypes, whereas RH 30 exhibited more number of shoot regeneration per explant but the frequency of shoot regeneration in this variety was poor than that of Varuna. The explants, cotyledon and plumule from Varuna produced the highest frequency (75% and 95%) of shoot regeneration, whereas both the explants from RH 30 produced highest number of shoots per explant. It is clear that different genotypes and explants have different potential for frequency of shoot regeneration as well as number of shoot regeneration per explant.